TOR:  RFP announcement for Carp GIP baseline health screening

Title: Search for expert diagnostic service laboratory to analyze biological samples for disease testing/reconfirmations of carp species from the WorldFish Carp Genetic Improvement Program (CGIP) and prepare primers to allow testing of additional samples by WorldFish and/or partners in Bangladesh.

Background: WorldFish is an international, non-profit research organization that harnesses the potential of fisheries and aquaculture to reduce hunger and poverty. In the developing world, more than 1 billion poor people obtain most of their animal protein from fish and 250 million depend on fishing and aquaculture for their livelihoods. The WorldFish mission is to strengthen livelihoods and enhance food and nutrition security by improving fisheries and aquaculture. We pursue this through research partner-ships focused on helping those who stand to benefit the most—poor producers and consumers, women and children.

The WorldFish CGIP aims to substantially increase aquaculture productivity in Bangladesh by developing and disseminating rapidly growing carp species to farmers. Genetically improved carp seed will be available in Bangladesh in late 2020. These fish are expected to grow 20%–30% more rapidly than currently available seed. WorldFish expects to further improve the growth rate by an average of 5%–10% every 2 years – the age at which new genetically superior parents can be selected and spawned. WorldFish is currently developing a dissemination strategy and business model to underpin a sustainable, genetically improved carp program for the country.

Goal: Assess CGIP baseline health by conducting polymerase chain reaction (PCR) tests for 10 pathogens, and next generation sequencing (NGS) for reconfirmation and to identify potentially harmful bacteria not included in the 10 pathogens.

Objectives:

Objective 1: PCR testing for 10 pre-defined pathogens – initial independent screening is to be undertaken by an accredited diagnostic service laboratory.  Additional samples are to be screened by WorldFish and/or partners using primers provided by the laboratory.

Disease/pathogen to screen for include:

1. Epizootic Ulcerative Syndrome (EUS)/Red Spot Disease (RSD)
2. Koi Herpes virus (KHV) disease, Cyprinid herpesvirus 3 (CyHV-3)
3. Spring viraemia of carp virus (SVCV)
4. Tilapia lake virus (TiLV)
5. Carp Edema Virus (CEV)
6. Infectious spleen and kidney necrosis virus (ISKNV)
7. Pseudomonas fluorescens
8. Aeromonas hydrophila

+2 additional pathogens to be decided

Objective 2: Bacterial microbiome analyses to identify potentially harmful bacteria not included in Objective 1 - amplicon sequencing by Next Generation Sequencing (NGS) using RPOB gene (better resolution than 16S)

Scope of Work: As part of the WorldFish CGIP and associated dissemination strategy, WorldFish has a responsibility to screen animals at the WorldFish Carp Genetic Improvement Facility near Jashore, making sure that they are free of significant pathogens. Currently there is very little capacity to test for carp pathogens in Bangladesh. To provide a comprehensive baseline assessment of the health status of fish maintained in the WorldFish Carp Genetic Improvement Facility, an expert diagnostic service laboratory is required to undertake initial PCR screening for 10 pre-defined pathogens, prepare primers for these 10 pathogens and conduct bacterial microbiome analyses to identify additional harmful bacteria

Activity

Deliverables

Start Date

End Date

Nucleic acid extractions, cDNA synthesis, PCR and analysis for 10 pathogens from 60 fish: 20 Rohu, 20 Catla and 20 silver carp

Complete report of PCR results for 10 pathogens from 60 samples

20th of July

End of July

Prepare primers and provide up-to-date and detailed PCR protocols for the 10 pathogens listed in Objective 1.

The provision of enough primers to conduct 1000 reactions for each of the 10 pathogens listed in Objective 1.  Detailed PCR protocols for the use of primers are to be made available online.  Each protocol will be accompanied by a short video (~2 minutes long).

15th of August

25th of August

Amplicon sequencing by NGS using RPOB gene. Include cost of DNA extraction, pre-PCR, library preparation, NGS, and analysis for 60 Fish.

Complete microbiome assemblage (bacteria present) from all key immune organs (pooled samples) for 60 fish

Identification of potential harmful bacteria that were not included in the list of 10 tested by PCR

20th of July

10th of August

Who can apply? (please mention what type of service/vendor you are loking for)

Accredited diagnostic service laboratories with molecular PCR and NGS capacity

Required documentation

If selected for the, the service/goods provider/vendor will be required to show:

  • A certificate of incorporation (for individual companies, a trade license);
  • Joint-stock registration certificate (if applicable);
  • An organizational organogram of key personnel, inclusive of the names of such personnel;
  • Tax Identification Number (TIN);
  • VAT registration number;
  • Proof of a segregated account (providing the name and address of such an account);
  • Other valid papers (provided by government institutions).
  • The consulting firm must bear all annual income tax (AIT).
  • The percentage of VAT and the total amount of VAT should be mentioned in the proposal.

Submission details: Interest bidder should submit their proposal before 13 July, 2020 via email to: procurement.wfbd@worldfishcenter.org; or, Sinthia.Shahrin@cgiar.org  mentioning the title of solicitation in the subject line.